Background:
In an effort to provide more updated reporting of reference intervals and in consultation with UCI’s Institute of Immunology, the Department of Pathology & Laboratory Medicine will change the adult reference intervals for T-cell, B-cell, and NK-cell subsets. These changes were verified in 50 adult (≥ 17 years old) donors as part of a reference range population study. Pediatric ranges remain the same.
| Markers |
Test Code |
PREVIOUS Adult |
NEW Adult |
| CD3 % |
TSUBS |
62-84 % |
58-89 % |
| CD3 Absolute Count |
TSUBS |
619-1847 |
700-2377 cells/mcL |
| CD3+CD4 % |
TSUBS |
31-61 % |
24-64 % |
| CD3+CD4 Absolute Count |
TSUBS |
338-1194 |
477-1634 cells/mcL |
| CD3+CD8 % |
TSUBS |
10-38 % |
12-45 % |
| CD3+CD8 Absolute Count |
TSUBS |
85-729 |
168-1315 cells/mcL |
| CD4/CD8 Ratio |
TSUBS |
0.9-3.7 |
0.8-5.0 |
| CD19 % |
BSUBS |
5-26 % |
6-29 % |
| CD19 Absolute Count |
BSUBS |
51-473 |
104-795 cells/mcL |
| CD56 % |
BSUBS |
1-17 % |
2-32 % |
| CD56 Absolute Count |
BSUBS |
12-349 |
43-680 cells/mcL |
Effective date: Dec. 1, 2019
Test codes: TSUBS, BSUBS
Specimen requirement and testing information:
Test methodology: Flow cytometry
Collect: 3.0 – 5.0 mL blood in lavender-top (EDTA) tube and 7.0 mL blood in green-top (sodium heparin) tube or 10.0 mL in yellow-top (ACD) tube.
Prepare: Specimen must arrive within 24 hours of draw. Send at ambient temperature. Do not refrigerate. Protect from heat.
Minimum volume: 0.5 mL blood in lavender-top (EDTA) tube and 2.0 mL blood in green-top (sodium heparin) or 1.0 mL in yellow-top (ACD) tube.
Minimum stability (collection to initiation of testing):
Ambient: EDTA = 24 hours
Na Heparin = 48 hours
ACD = 72 hours
Refrigerated: Unacceptable
Frozen: Unacceptable
Turnaround time (TAT):
STAT: Not available
Routine: 1 - 3 days
Set Up Days: Monday - Saturday
Rejection criteria:
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, and specimens submitted in leaking containers, clotted or grossly hemolyzed specimens
Additional information:
Useful for the quantitation of T-helper (CD4) and T-suppressor (CD8) and total T cell (CD3) lymphocytes and determination of CD4:CD8 ratio.
A summary of all tests offered by our laboratory services can be found here: http://www.pathology.uci.edu/services/index.asp
Sincerely,
Xiaohui (Sheila) Zhao, MD, PhD
Director
Hematology Laboratory
Edwin S. Monuki, MD, PhD
Chair
Department of Pathology & Laboratory Medicine
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