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Enterovirus by PCR (CSF): In-house Test Discontinuation
The Microbiology Division of the Department of Pathology & Laboratory Medicine will no longer offer the Enterovirus by PCR in-house testing. The current in-house test - Cepheid Xpert® EV assay - is no longer being manufactured. The decision to discontinue Xpert EV was made by Cepheid after careful consideration of its ability to reliably manufacture the product, genetic drift within the target organism, and supplier uncertainty related to key manufacturing components.

Enterovirus by PCR can still be ordered; samples will be forwarded to our reference laboratory, ARUP. Results will not be available STAT, but will generally be available in two to three days.

Effective date: Jan. 27, 2020

Discontinued test code: SEVPCR [Enterovirus PCR (CSF)]
New test code: XENTPC (Enterovirus PCR)

Specimen requirements for sendout test:
Collect: CSF (sterile container). Also acceptable: Lavender (EDTA), pink (K2EDTA), serum separator tube and nasopharyngeal swab
Preparation: spin down and separate serum or plasma
Specimen required: 1.0 mL CSF, serum or plasma. Swab: place in viral transport media (ARUP Supply #12884).
Minimum volume: 0.5 mL

Ambient: 8 hours
Refrigerated: 48 hours
Frozen: 3 months

STAT: Not available
Turnaround time (TAT): 2 – 3 days

Rejection criteria:
Specimens that exceed stated stability, unlabeled/mislabeled/mismatched specimens, specimens submitted in leaking containers, non-sterile containers, heparinized specimens.

For additional information:​​​


Cassiana Bittencourt, MD
Division of Clinical Microbiology

Edwin S. Monuki, MD, PhD
Department of Pathology & Laboratory Medicine
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